MtSSPdb EXPERIMENT INFORMATION


The SSP Gene Expression Atlas is part of the MtSSPdb, and hosts in total 14 RNA-seq experiments represented by 433 samples and 129 conditions.

Hormone Treatment RNA-seq libraries were obtained from root tissues of M. truncatula A17 plants three days post germination. The seedlings were immersed in B&D full nutrient media (Ph 6.8) containing the hormones at different concentrations; 1 ┬ÁM IAA (Auxin), 100 nM BAP (Cytokinin) and an equal volume of the solvent control (DMSO). The seedilngs were placed in the dark for three hours after which the root tips were removed and 40-60 roots per replicate were used.
Macronutrient Deficiencies 1 Publicly available RNA-seq dataset from de Bang et al. 2017 (DOI: 10.1104/pp.17.01096). RNA-seq libraries were prepared from root and shoot tissues of plants grown under sulfur (S), potassium (K), nitrogen (N) and phosphorus depleted conditions. The response to persistent deficiency was tested by growing the plants for 3 weeks under full nutrition (FN) or under deficiency of N, P, K or S. Additional samples were collected from plants re-supplied with FN for 6 h following the nutrient-deficiency treatment.
Macronutrient Deficiencies 2 Publicly available RNA-seq dataset from de Bang et al. 2017 (DOI: 10.1104/pp.17.01096). RNA-seq libraries were prepared from root and shoot tissues of plants grown under full nutrition, and with no nitrogen or phosphorus collected after 22 days of germination.
Nodule Development 1 Publicly available RNA-seq dataset from de Bang et al. 2017 (DOI: 10.1104/pp.17.01096). RNA-seq libraries were obtained from root/nodule tissues at varying developmental stages of nodulation with Sinorhizobium meliloti. The stages were: pre-inoculated root; root at 4 dpi, harboring the initial nodule bump during initiation; nodule at 10 dpi (expanding nodule); nodule at 14 dpi (actively-fixing nodule), and 28 dpi (senescing nodule). Additional samples were collected from nodules that were re-supplied with nitrogen at 14 dpi, to suppress nitrogen fixation.
Nodule Development 2 Publicly available RNA-seq dataset from Roux et al. 2014 (DOI: 10.1111/tpj.12442). Samples were collected from root nodules at 10 or 15 dpi after inoculation with S. meliloti.
Nodule Development 3 Publicly available RNA-seq datasets from Larrainzar et al. 2015 (DOI: 10.1104/pp.15.00350) were collected from a time-course of rhizobial infection events within the first 48 hours post-inoculation (hpi).
Nodule Development 4 Publicly available RNA-seq datasets from Satge et al 2016 (DOI: h10.1038/nplants.2016.166). M. truncatula plants (pDME-DMEi and pDME-GUS - control) were inoculated with Rhizobia meliloti.
Plant Tissues RNA-seq libraries were prepared from different plant tissues: root, leaf, leaf bud, pod, flower, petiole, and bare stalk collected at different stages.
Salt stress RNA-seq libraries were prepared from root and shoot tissues of plants grown under salt treatment (NaCl) collected after 25 days.
Symbiotic interactions 1 Publicly available RNA-seq datasets from Camps et al. 2015 (DOI: 10.1111/nph.13427) were collected from P-starved root tissue treated with sulfated or non-sulfated Myc-LCOs for three hours.
Symbiotic interactions 2 Publicly available RNA-seq datasets from Garcia et al. 2017 (DOI: 10.1104/pp.16.01959) were collected from M. truncatula roots inoculated or not with the arbuscular mycorrhizal fungus Rhizophagus irregularis and watered with a high (3.75 mM) or low (0.05 mM) potassium solution for 6 weeks.
Symbiotic interactions 3 Publicly available RNA-seq datasets from Jardinaud et al. 2016 (DOI: 10.1104/pp.16.00711) were collected from root epidermis after 4 or 24h treatment with Nod factors or mock treatment in n order to analyze very early symbiotic stages.
Symbiotic interactions 4 Publicly available RNA-seq datasets from Luginbuehl et al. 2017 (DOI 10.1126/science.aan0081) were collected from M. truncatula roots inoculated or not with the arbuscular mycorrhizal fungus Rhizophagus irregularis (8, 13 and 27 dpi).
Symbiotic interactions 5 Publicly available RNA-seq datasets from Zeijl et al. 2015 (DOI http://dx.doi.org/10.1016/j.molp.2015.03.010) were collected from M. truncatula roots inoculated with Rhizobia meliloti.